Pentr topo d manual

ViraPower™ Lentiviral Directional TOPO® Expression Kit KpLenti6/ V5 Directional TOPO® Cloning Kit KKit Components Catalog nos. The pENTR/ D- TOPO Cloning Kits utilize a highly efficient, 5- minute cloning strategy ( TOPO Cloning) to directionally clone a blunt- end PCR product into a vector for entry into the Gateway System or the MultiSite Gateway System. I have been trying to generate clones using pENTR DTOPO for gateway cloning. Coli 20 reactions Life Sciences: Molecular Biology Reagents. Kit Size Catalog no. PENTR™ / D- TOPO® Cloning Kit ® with One Shot TOP10 Chemically Competent E.

PENTR™ Directional TOPO® Cloning Kits Five- minute, directional TOPO® Cloning of blunt- end PCR products into an entry vector. Into the vector and cut itself out. The pENTR/ SD/ D- TOPO Cloning Kits utilize a highly efficient, 5- minute cloning strategy ( TOPO Cloning) to directionally clone a blunt- end PCR product into a vector for entry into the Gateway System or the MultiSite Gateway System. Description: Gateway type ( lambda att- type) recombinational cloning system entry ( master) vector for TOPO cloning of insert; kanamycin.

Materials needed: pENTRTM Directional TOPO® Cloning Kit ( # Kby Invitrogen. Not intended for any animal or human therapeutic or diagnostic use. PENTR™ / D- TOPO® Cloning Kit with One Shot® TOP10 Chemically. According to the manual: " To obtain the highest TOPO® Cloning efficiency, use a 0. Constitutive: Unspecified. These TOPO reactions are by far not as efficient as described in the manual.


Product Catalog no. Coli 20 reactions 20 reactions KKpENTR™ / SD/ D- TOPO® Cloning Kit with One Shot® TOP10 Chemically Competent E. K4300- 01, KVersion N 13 AprilA Limited Use Label License covers this product ( see Purchaser Notification). Vector Database Homepage; pENTR/ D- TOPO; Vector Database. Vii Kit Contents and Storage Types of Kits This manual is supplied with the following products. Bacterial Resistance: Kanamycin.

User guide For Research Use Only. Vii Kit Contents and Storage Types of Kits This manual is supplied with the following kits. Product overview. Recommend that you follow the detailed protocols provided in the manual. Terms and Conditions © Harvard Medical School PlasmID was created and is maintained by the DF/ HCC DNA Resource Core at Harvard Medical SchoolDF/ HCC DNA Resource Core at.

I am doing cloning a gene ( 1338 bp) into pENTR/ D- TOPO vector. Coli Catalog nos. Citations & references. Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. Blunt- end PCR products clone directionally at greater than 90% efficiency.
I' ve got a problem with Invitrogen' s pENTR D TOPO cloning kit. Invitrogen Gateway pENTR/ D- TOPO Cloning Kit, with One Shot TOP10 Chemically Competent E. PENTR™ 1A VectorpENTR™ 2B VectorpENTR™ 3C VectorpENTR™ 4 VectorpENTR™ 11 VectorShipping and.
Problems with pENTR/ D- TOPO, is possible that my insert isn' t cloned full? Instruction Manual pBAD TOPO® TA Expression Kit Five- minute cloning of Taq polymeraseamplified PCR products for regulated expression in E. Created with Raphaël 2601 bpApaLIpBR322_ origin NruIORF frame 2 KanR2. Blunt- end PCR products clone directionally into the pENTR/ SD/ D- TOPO en.

Coli ™ with One Shot® Mach1 - T1R Chemically Competent E. Name: pENTR/ D- TOPO. I am using a protocol from the manual of the TOPO cloning kit. C) I then follow the instructions and transform the entry clone into chemically. Pentr topo d manual. The pENTR/ D- TOPO Cloning Kits utilize a highly efficient, 5- minute cloning strategy ( TOPO Cloning) to directionally clone a blunt- end PCR product into a vector. This is a free resource for the scientific community that is compiled by Addgene. This manual is supplied with the following kits.
Gateway Cloning - pENTER D TOPO. 5µl pENTR/ D- TOPO. Important Information pENTR™ Vectors This manual is supplied with the following products.


Notes: Directional TOPO cloning vector. For a detailed description of the reagents supplied with the pLenti6/ V5- D- TOPO. The manual has been written in a protocol format, with little theoretical discussion. The pENTR/ D- TOPO Cloning Kits utilize a highly efficient, 5- minute cloning.

Invitrogen Gateway pENTR/ D- TOPO Cloning Kit, with One Shot TOP10 Chemically Competent E. Kand Kinclude the following components. Digest the plasmid with NotI/ AscI to see whether your insert is cloned into the TOPO plasmid? And fused in frame with the TEV recognition site ( in pENTR™ / TEV/ D- TOPO®.


Stable: Unspecified. Catalog Number: K2400- 20. ( Check the manual for more details).



Phone:(771) 738-3955 x 7781

Email: [email protected]